Part:BBa_K1606029:Design

Gluten Updated m-cherry reporter gene

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 567
  Illegal AgeI site found at 679
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga

gttcgaaatcgaaggtgaaggtg

aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac

atcctgtccccgcagttccagta

cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaat

gggaacgtgttatgaacttcgaa

gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg

taccaacttcccgtccgacggtc

cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt

gaaatcaaaatgcgtctgaaact

gaaacca caa ctt ccagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa

aaccgacatcaaactggac

atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctta

ataacgctgatagtgctagtgta

gatcgc

Source

It comes from genomic sequence and we add a sequence that square with the amino acid sequence of the gluten protein of PQLP amino acid sequence that is digested by Kumamax enzyme.